Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked
نویسندگان
چکیده
منابع مشابه
Tn916-generated, lipooligosaccharide mutants of Neisseria meningitidis and Neisseria gonorrhoeae.
A library of Tn916-generated, tetracycline-resistant (Tc) mutants of the group B Neisseri meningitidis strain NMB was screened by using monoclonal antibodies (MAbs) that recognize structural differences in neisserial lipooligosaccharide (LOS). The LOS of parental strain NMB had a relative molecular mass of 4.5 kDa, reacted with MAbs 3F11 and 6B4 but not with MAb 4C4 or 6E4, and contained a lact...
متن کاملLRLW-LSI: An Improved Latent Semantic Indexing (LSI) Text Classifier
The task of Text Classification (TC) is to automatically assign natural language texts with thematic categories from a predefined category set. And Latent Semantic Indexing (LSI) is a well known technique in Information Retrieval, especially in dealing with polysemy (one word can have different meanings) and synonymy (different words are used to describe the same concept), but it is not an opti...
متن کاملRole of phosphoglucomutase in lipooligosaccharide biosynthesis in Neisseria gonorrhoeae.
A region of pSG30 that complements the pyocin-derived gonococcal lipooligosaccharide (LOS) mutants 1291d and 1291e was characterized by DNA sequence analysis and an open reading frame of 1,380 bases was identified that is 89% similar and 56% identical over 452 amino acids to the algC gene product from Pseudomonas aeruginosa that encodes phosphomannomutase. Enzymatic analysis of gonococcal crude...
متن کاملLatent Semantic Indexing (LSI): TREC-3 Report
We used LSI for both TREC-3 routing and adhoc tasks. For the routing tasks an LSI space was constructed using the training documents. We compared profiles constructed using just the topic words (no training) with profiles constructed using the average of relevant documents (no use of the topic words). Not surprisingly, the centroid of the relevant documents was 30% better than the topic words. ...
متن کاملMolecular analysis of lipooligosaccharide biosynthesis in Neisseria gonorrhoeae.
A HindIII gene bank of Neisseria gonorrhoeae MUG116 was constructed in the cosmid vector pHC79. A cosmid (pSY81) was isolated that was able to convert N. gonorrhoeae FA5100 to reactivity with monoclonal antibody (MAb) 2-1-L8. Several MAb-reactive transformants were isolated and characterized with respect to lipooligosaccharide (LOS) production as analyzed by sodium dodecyl sulfate-polyacrylamid...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1994
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.176.11.3428-3432.1994